DNA vaccination for cervical cancer; a novel technology platform of RALA mediated gene delivery via polymeric microneedles

In this study, we have developed a technology for two two-step delivery of DNA vaccine for cervical cancer. We have utilized the RALA peptide to deliver the E6/E7 plasmid in a dissolving microneedle patch (MN). During the fabrication process, the RALA-E6/E7 nanoparticles are loaded into the microneedles, and left 48 h to dry. Following in vivo application of the MN/RALA-E6/E7 vaccine, we successfully demonstrated more consistent humoral mediated immune response, and the ability of the vaccine to evoke both a prophylactic and therapeutic response to tumor challenge.118