Evaluation of expressed prostatic secretion and serum using surface-enhanced Raman spectroscopy for the noninvasive detection of prostate cancer, a preliminary study

Expressed prostatic secretion was obtained through noninvasive procedure-gentle prostate massage during digital rectal examination (DRE) from patients. SERS spectra of EPS and serum was collected by Raman microscope. Significant differences in spectra between prostate cancer and benign prostatic hyperplasia were tentatively assigned to component changes in EPS and serum samples. Principal component analysis(PCA) and linear discriminate analysis(LDA) were utilized to evaluate the spectral data for EPS and serum, to build diagnostic algorithms. The results demonstrated that SERS analysis of EPS could be a potential meaningful clinical adjunct for PCa detection.79