کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5043362 1475139 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo two-photon imaging of striatal neuronal circuits in mice
ترجمه فارسی عنوان
تصویر برداری دو فوتون در مدارهای نورونی مانیتور در موشها در داخل
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب رفتاری
چکیده انگلیسی


- Striatal GABAergic neurons are fluorescently labeled by AAV9 vectors or in transgenic mice.
- Direct two-photon imaging through an implanted window visualizes dorsal striatal neurons in vivo.
- High-resolution imaging visualizes the dendritic spines of striatal neurons.

Imaging studies of the subcortical striatum in vivo have been technically challenging despite its functional importance in movement control and procedural learning. Here, we report a method for imaging striatal neuronal circuits in mice in vivo using two-photon microscopy. Cell bodies and intermingled dendrites of GABAergic neurons labeled with fluorescent proteins were imaged in the dorsal striatum through an imaging window implanted in the overlying cortex. This technique could be highly useful for studying the structure and function of striatal networks at cellular and subcellular resolutions in normal mice, as well as in mouse models of neurological disorders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Learning and Memory - Volume 135, November 2016, Pages 146-151
نویسندگان
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