کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5119775 1485973 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lipoic acid protects gastric mucosa from ethanol-induced injury in rat through a mechanism involving aldehyde dehydrogenase 2 activation
ترجمه فارسی عنوان
اسید لیپوئیک از مخاطی معده از آسیب ناشی از اتانول در موش صحرایی از طریق مکانیسم فعال سازی آلدهید دهیدروژناز 2 محافظت می کند
کلمات کلیدی
اسید لیپوئیک، آلدهید دهیدروژناز 2، 4-هیدروکسی -2-غیر انالال، اتانول، مخاطی معده،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- ALDH2 activity in gastric tissues was decreased after ethanol treatment.
- Reactive aldehydes in gastric tissues were accumulated after ethanol treatment.
- Lipoic acid exerted beneficial effects on ethanol-induced mucosal injury.
- Lipoic acid attenuated ethanol - induced reactive aldehydes accumulation.
- Lipoic acid elevated ALDH2 activity in enthanol-treated gastric tissue.

Numerous studies demonstrate that reactive aldehydes are highly toxic and aldehyde dehydrogenase 2 (ALDH2)-mediated detoxification of reactive aldehydes is thought as an endogenous protective mechanism against reactive aldehydes-induced cell injury. This study aims to explore whether lipoic acid, a potential ALDH2 activator, is able to protect gastric mucosa from ethanol-induced injury through a mechanism involving clearance of reactive aldehydes. The rats received 60% of acidified ethanol through intragastric administration and held for 1 h to establish a mucosal injury model. Lipoic acid (10 or 30 mg/kg) or Alda-1 (a positive control, 10 mg/kg) was given 45 min before the ethanol treatment. The gastric tissues were collected for analysis of gastric ulcer index, cellular apoptosis, 4-hydroxy-2-nonenal (4-HNE) and malondialdehyde (MDA) contents, and ALDH2 activity. The results showed that acute administration of ethanol led to an increase in gastric ulcer index, cellular apoptosis, 4-HNE and MDA contents concomitant with a decrease in ALDH2 activity; these phenomena were reversed by lipoic acid or Alda-1. The gastric protection of lipoic acid was attenuated in the presence of ALDH2 inhibitor. Based on these observations, we conclude that lipoic acid exerts the beneficial effects on ethanol-induced injury through a mechanism involving, at least in part, ALDH2 activation. As a dietary supplement or a medicine already in some countries, lipoic acid can be used to treat the ethanol - induced gastric mucosal injury.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Alcohol - Volume 56, November 2016, Pages 21-28
نویسندگان
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