کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5132406 | 1492048 | 2018 | 6 صفحه PDF | دانلود رایگان |
- Using H2O2 under alkaline condition (pHÂ 10) is a new method to prepare POS.
- POS samples are potential prebiotic candidates, especially POSH1.
- POSH1 shows the highest prebiotic score for Bifidobacterium bifidum ATCC29521 (0.92).
Pectin oligosaccharide (POS) fractions were obtained by controlled chemical degradation of citrus peel pectin. By adjusting trifluoroacetic acid (TFA) concentration, three oligosaccharides were obtained, of molecular weight (Mw) range 3000-4000â¯Da, 2000-3000â¯Da and lower than 2000â¯Da. Varying hydrogen peroxide (H2O2) concentration and reaction time produced oligosaccharides of 3000-4000â¯Da and 2000-3000â¯Da. The relative proportions of acidic monosaccharide increased from 68.58% to 89.93% (TFA) and from 63.74% to 83.26% (H2O2) as the reaction conditions intensified. Prebiotic activity scores were used to quantify POS ability to promote selective growth of specific probiotics. Sample POSH1 from H2O2 degradation showed the highest prebiotic potential with prebiotic activity score 0.41 for Lactobacillus paracasei LPC-37 and 0.92 for Bifidobacterium bifidum ATCC 29521.
Journal: Food Chemistry - Volume 244, 1 April 2018, Pages 232-237