کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5132648 | 1492050 | 2018 | 8 صفحه PDF | دانلود رایگان |
- Straightforward method by LC-MS/MS to determine opium alkaloids in poppy seeds.
- The scope of the method covers morphine, codeine, thebaine, noscapine, papaverine and narceine.
- Morphine, codeine and thebaine were detected in 100%, 78% and 78% of the samples.
- 60% of the samples exceed the German guidance value of 4Â mg/kg of morphine set in Germany.
A straightforward method to determine the content of six opium alkaloids (morphine, codeine, thebaine, noscapine, papaverine and narceine) in poppy seeds and bakery products was developed and validated down to a limit of quantification (LOQ) of 0.1Â mg/kg. The method was based on extraction with acetonitrile/water/formic acid, ten-fold dilution and analysis by LC-MS/MS using a pH 10 carbonate buffer.The method was applied for the analysis of 41 samples collected in 2015 in the Netherlands and Germany. All samples contained morphine ranging from 0.2 to 240Â mg/kg. The levels of codeine and thebaine ranged from below LOQ to 348Â mg/kg and from below LOQ to 106Â mg/kg, respectively. Sixty percent of the samples exceeded the guidance reference value of 4Â mg/kg of morphine set by BfR in Germany, whereas 25% of the samples did not comply with the limits set for morphine, codeine, thebaine and noscapine by Hungarian legislation.
Journal: Food Chemistry - Volume 242, 1 March 2018, Pages 443-450