Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIA

• Chicken IgY antibody based immunoassays as alternative strategy in antibiotic detection.
• Two specific IgY antibodies against gentamicin (Gent) and kanamycin (Kana) were prepared.
• Good agreement of IgY antibody-based aminoglycoside antibiotics detection and two immunoassays.

Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC50 of FPIA and ic-ELISA based anti-Gent IgY were 7.70 ± 0.6 μg/mL and 0.32 ± 0.06 μg/mL, respectively. The IC50 of FPIA and ic-ELISA based anti-Kana IgY were 7.97 ± 0.9 μg/mL and 0.15 ± 0.01 μg/mL. The limits of detection (LOD, IC10) for FPIA based anti-Gent/Kana IgY were 0.17 and 0.007 μg/mL, respectively. The LOD for ic-ELISA were both 0.001 μg/mL. These results indicated that the ic-ELISA might more suitable for antibiotic residues detection than FPIA.