کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5133473 | 1492060 | 2017 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development of a competitive immunochromatographic assay for the sensitive detection of amantadine in chicken muscle Development of a competitive immunochromatographic assay for the sensitive detection of amantadine in chicken muscle](/preview/png/5133473.png)
- CGICA was established for the detection of AMD in chicken muscle.
- CGICA had high sensitivity at 1.80Â ng/mL.
- The CGICA results were highly correlated with the ELISA and UPLC-MS/MS results.
- CGICA is suitable for the rapid detection of AMD in chicken muscle on-site.
Amantadine (AMD) is a prohibitive veterinary medicine in the entire world. In this study, a sensitive colloidal gold immunochromatographic assay (CGICA) was established for the rapid semi-quantitative detection of AMD in chicken muscle. Under optimal conditions, the detection results were obtained in 12Â min with a limit of detection for 1.80Â ng/mL. CGICA presented a good linear range from 2.5Â ng/mL to 25Â ng/mL, with only 11.5% cross-reactivity with rimantadine. The recovery rates for the fortified samples were ranged from 81% to 120%. The coefficient of variation of the intra-assay and inter-assay was less than 15%. The accuracy of CGICA was confirmed by systematically comparing the result of the proposed method with enzyme-linked immunosorbent assay and ultra-high-performance liquid chromatography-tandem mass spectrometry. Given the advantages of its simplicity, convenience, and speediness, the proposed CGICA is suitable for the on-site rapid detection of AMD in chicken muscle.
Journal: Food Chemistry - Volume 232, 1 October 2017, Pages 770-776