کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5133530 | 1492069 | 2017 | 7 صفحه PDF | دانلود رایگان |
- A simple and reproducible analytical on-line method for the determination of PAHs was developed.
- The on-line methodology showed adequate accuracy and precision for analysis of PAHs in foods.
- The LODs and LOQs for all PAHs were 0.012 ng gâ1 and 0.04 ng gâ1 respectively.
- MSPD-SPE-HPLC/FLD method used only 50Â mg of sample and low consumption of reagents.
- The methodology presents advantages with respect other expensive analysis systems.
A fast method was optimized and validated for simultaneous trace determination of four polycyclic aromatic hydrocarbons: benzo[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene and benzo[a]pyrene in bovine tissues. The determination was performed by matrix solid-phase dispersion (MSPD) coupled on-line to solid phase extraction (SPE) and high performance liquid chromatography (HPLC) with fluorescence detection (FLD). The sample was dispersed on C18 silica sorbent and then the on-line MSPD-SPE-HPLC/FLD method was applied. Several parameters were optimized: cleaning and elution sequences applied to the MSPD cartridge, the flow rate and dilution of extract used for SPE loading. The on-line method was validated over a concentration range of 0.1-0.6 ng gâ1 obtaining good linearity (r ⩾ 0.998) and precision (RSD) ⩽ 10%. Recovery ranged from 96 to 99% and the limits of detection were 0.012 ng gâ1. This methodology was applied to liver samples from unhealthy animals. The results demonstrate that MSDP-SPE-HPLC/FLD method provides reliable, sensitive, accurate and fast data to the food control.
Journal: Food Chemistry - Volume 223, 15 May 2017, Pages 82-88