Trace determination of organophosphate esters in white wine, red wine, and beer samples using dispersive liquid-liquid microextraction combined with ultra-high-performance liquid chromatography-tandem mass spectrometry

- DLLME-UPLC-MS/MS was developed for the determination of OPEs from wines.
- Results showed that high analytes recoveries were achieved with the proposed method.
- The tested OPEs were found in the real samples with different levels.
- It is promising to be used for the analysis of trace amount of OPEs in the future.

In this study, dispersive liquid-liquid microextraction coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry was developed for the analysis of five representative organophosphate esters (OPEs) in wine samples. Under optimized conditions, the proposed method resulted in good linearity (R2 > 0.9933) over the range of 0.1-100 μg L−1, with limits of detection (LODs, S/N = 3) and quantification (LOQs, S/N = 10) in the ranges of 0.48-18.8 ng L−1 and 1.58-62.5 ng L−1, respectively. Inter- and intra-assay precisions of RSD% ranged from 3.21% to 6.13% and from 1.69% to 7.63%, respectively. The spiked recoveries of target OPEs from white wine, red wine, and beer samples were in the ranges of 80-122%, 76-120%, and 76-110%, respectively, at two different concentration levels. The total concentrations of five OPEs found in white wine, red wine, and beer samples were in the ranges of 0.29-0.85 μg L−1, 1.00-3.05 μg L−1, and 0.86-1.47 μg L−1, respectively.