کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5134173 1492073 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel isoflavone profiling method based on UPLC-PDA-ESI-MS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A novel isoflavone profiling method based on UPLC-PDA-ESI-MS
چکیده انگلیسی


- Application of UPLC-PDA-ESI-MS in non-targeted isoflavone profiling.
- Evidence for structural variants of positional isomers using UPLC-ESI-MS.
- Optimization of three possible quantitative methods in one run.

A novel non-targeted isoflavone profiling method was developed using the diagnostic fragment-ion-based extension strategy, based on ultra-high performance liquid chromatography coupled with photo-diode array detector and electrospray ionization-mass spectrometry (UPLC-PDA-ESI-MS). 16 types of isoflavones were obtained in positive mode, but only 12 were obtained in negative mode due to the absence of precursor ions. Malonyldaidzin and malonylgenistin glycosylated at the 4′-O position or malonylated at the 4″-O position of glucose were indicated by their retention behavior and fragmentation pattern. Three possible quantification methods in one run based on UPLC-PDA and UPLC-ESI-MS were validated and compared, suggesting that methods based on UPLC-ESI-MS possess remarkable selectivity and sensitivity. Impermissible quantitative deviations induced by the linearity calibration with 400-fold dynamic range was observed for the first time and was recalibrated with a 20-fold dynamic range. These results suggest that isoflavones and their stereoisomers can be simultaneously determined by positive-ion UPLC-ESI-MS in soymilk.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 219, 15 March 2017, Pages 40-47
نویسندگان
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