کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5139039 1494861 2017 40 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High-performance liquid chromatography with fast-scanning fluorescence detection and post-column on-line photoderivatization for the analysis of folic acid and its metabolites in vegetables
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
High-performance liquid chromatography with fast-scanning fluorescence detection and post-column on-line photoderivatization for the analysis of folic acid and its metabolites in vegetables
چکیده انگلیسی
The photochemical behavior of folic acid and its metabolites, tetrahydrofolic and 5-methyltetrahydrofolic acids has been followed by high performance liquid chromatography, coupled with a fast scanning fluorimetric detector (HPLC-FSFD). The main photoproducts generated were characterized through accurate mass measurement using liquid chromatography-mass spectrometry (HPLC-MS). The effect on photogeneration processes of irradiation time, and the hydrogen peroxide concentration, in presence and in absence of N2 stream, was also studied. A chromatographic method with post-column on-line photoderivatization has been developed for the analysis of folic, tetrahydrofolic and 5-methyltetrahydrofolic acids. Linearity ranges from 50 to 1000 ng·mL− 1 for folic acid, and from 100 to 1000 ng·mL− 1 for tetrahydrofolic and 5-methyltetrahydrofolic acids have been established. Detection limits of 7.5 ng·mL− 1, 15.3 ng·mL− 1 and 22.9 ng·mL− 1 were calculated for folic acid, tetrahydrofolic acid and 5-methyltetrahydrofolic acid respectively. The method has been satisfactorily applied to cereals, spinach and tomato analysis. Serum samples analysis was also tested.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 133, July 2017, Pages 333-345
نویسندگان
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