Spectrometric assay for horseradish peroxidase activity based on the linkage of conjugated system formed by oxidative decarboxylation

Colorimetric (absorption at 483 nm) and fluorometric (emission at 529 nm) determination of HRP using BAFA as a hydrogen donor both gave a linear calibration curve (y = 0.0025 × + 0.0237; R2 = 0.9997, y = 0.241 × + 3.194; R2 = 0.9914) in the same range of 1.4-12 U/L.270