High yield synthesis of cyclic analogues of antibacterial peptides P-113 by Sortase A-mediated ligation and their conformation studies

Four cyclized antibacterial peptides P-113 analogs were designed and synthesized by Sortase A-mediated ligation. The conformation studies by CD spectrum in aqueous buffers and in trifluroethanol (TFE) suggested that α-helix structures were produced progressively in hydrophobic environment independent of the cyclization, which displayed the similar behavior as parent peptide P-113.