Production of trehalase inhibitor validoxylamine A using acid-catalyzed hydrolysis of validamycin A

As a competitive trehalase inhibitor and lead compound with broad uses, validoxylamine A has been increasingly studied for its many possible applications. The process of acid-catalyzed hydrolysis of validamycin A was investigated for production of validoxylamine A. The effects of various acids and substrate concentrations were explored, and the mechanism of acid-catalyzed hydrolysis of validamycin A was proposed. Results demonstrated that the optimal condition of acid hydrolysis of validamycin A included 1 M H2SO4 and a 30% (w/v) initial validamycin A concentration in a 1 h reaction time.