Characterization of immobilization methods of antiviral antibodies in serum for electrochemical biosensors

► A comparative study of different immobilizations of serum antibodies on the silanized surface was performed for optimizing electrochemical detection. ► Protein A revealed as a good bio-molecule for improvement and orientation of serum antibodies on the silanized surface. ► The most effective method of serum antibodies immobilization was tested on the protein A/ glutaraldehyde/ silanized interdigitated surface. ► The potential development of serum antibodies based biosensors could be realized for rapid and direct detection of viral antigens.