Immobilization of oligonucleotide probes on silicon surfaces using biotin-streptavidin system examined with microscopic and spectroscopic techniques

- Comparing 4 approaches to immobilize biotinylated oligonucleotide using streptavidin.
- Pre-adsorbed biotinylated BSA provides higher oligonucleotide surface density.
- ToF-SIMS reveals multi-molecular composition of organic overlayer on silicon surface.
- Composition accords with molecular coverage (ARXPS) and average height (AFM).