The effects of general anesthetics on ESR spectra of spin labels in phosphatidylcholine vesicles containing purified Na,K-ATPase or microsomal protein

► We studied the effects of general anesthetics on liposome using ESR spectra. ► Two spin labels, 5-DSA and 16-DSA, were located in different position in liposome. ► Anesthetics did not change the environment around the spin labels in the liposome. ► Anesthetics remained on the surface of the lipid bilayer of liposome. ► Proteins in the liposome did not change the effects of anesthetics on liposome.