کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5365092 | 1388325 | 2008 | 4 صفحه PDF | دانلود رایگان |
Biomaterials that support vascularization without activating immune competent endothelial cells are desirous in immunoisolation. The aim of the present study was to evaluate effect of chitosan-alginate membrane on adhesion and activation of endothelial cells. The AFM photographs showed that the CS/ALG membrane surface roughness values were less than CS membrane and ALG membrane. The contact angle of CS/ALG membrane was between ALG membrane and CS membrane. The surface roughness and hydrophilicity of CS/ALG membrane affect the adhesion and activation on endothelial cells. The CS/ALG membrane did not allow the majority of cells to adhere well but maintained their viability. The membrane leach-outs were nontoxic to the cells, as confirmed by tetrazolium reduction (MTT) and trypan blue assays. The growth curves of the endothelial cells desquamated from the CS/ALG membrane suggested that the endothelial cells maintain their activation. Taken together these results point out that CS/ALG membrane is compatible with endothelial cells and maintains their nonactivated status.
Journal: Applied Surface Science - Volume 255, Issue 2, 15 November 2008, Pages 422-425