Enzymes immobilization on Fe3O4-gold nanoparticles

In the present study Fe3O4 magnetic nanoparticles were synthesized by coprecipitation of Fe2+ and Fe3+ from chlorides. In the next step magnetite-gold core-shell nanoparticles were synthesized from HAuCl4 using an ethanol as a reducing agent. Finally, magnetic nanoparticles were functionalized by hexadecanethiol. The immobilization of biological molecules (trypsin and glucose oxidase) to the thiol-modified and unmodified magnetite-gold nanoparticles surface was tested. The resulting nanoparticles were characterized by infrared spectroscopy, differential scanning calorimetry, Mössbauer spectroscopy and transmission electron microscopy.

► Core-shell magnetic nanoparticles were biofunctionalized. ► Trypsin and glucose oxidase was successfully immobilized at the particle surface. ► Characterization of obtained biocomposites was done by IR, DSC, XRD, TEM and Mossbauer spectroscopy.