کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5366545 | 1388350 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Enzymes immobilization on Fe3O4-gold nanoparticles Enzymes immobilization on Fe3O4-gold nanoparticles](/preview/png/5366545.png)
In the present study Fe3O4 magnetic nanoparticles were synthesized by coprecipitation of Fe2+ and Fe3+ from chlorides. In the next step magnetite-gold core-shell nanoparticles were synthesized from HAuCl4 using an ethanol as a reducing agent. Finally, magnetic nanoparticles were functionalized by hexadecanethiol. The immobilization of biological molecules (trypsin and glucose oxidase) to the thiol-modified and unmodified magnetite-gold nanoparticles surface was tested. The resulting nanoparticles were characterized by infrared spectroscopy, differential scanning calorimetry, Mössbauer spectroscopy and transmission electron microscopy.
⺠Core-shell magnetic nanoparticles were biofunctionalized. ⺠Trypsin and glucose oxidase was successfully immobilized at the particle surface. ⺠Characterization of obtained biocomposites was done by IR, DSC, XRD, TEM and Mossbauer spectroscopy.
Journal: Applied Surface Science - Volume 258, Issue 7, 15 January 2012, Pages 2783-2787