کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5367932 | 1388378 | 2011 | 5 صفحه PDF | دانلود رایگان |
The binding equilibrium of potassium perfluorooctanesulfonate (FC95) to DNA was studied by UV spectra and resonance light scattering technique in the present paper. It was found that the interaction resulted in strongly enhanced resonance light scattering signals characterized by the RLS peak at 370Â nm. The results indicated that the conformation of the DNA has changed during the interaction because the micro environment of DNA changed. The binding constant Kf and the number of binding n were calculated according to the equilibrium equation, which was established based on the data of the resonance light scattering measurements at 370Â nm. It was found that the binding Kf and the number of binding n were 6.02Â ÃÂ 103 and 2.0, respectively.
Research highlightsⶠFC95 was used to determine DNA. ⶠRLS technique is used in comprehension of the binding of PFOS to DNA. ⶠThe mechanisms of the interaction was studied by UV spectra and RLS spectra.
Journal: Applied Surface Science - Volume 257, Issue 10, 1 March 2011, Pages 4547-4551