Quantitative analysis of end-to-end distance of single polymer chain in ultra-thin film by super-resolution fluorescence imaging

► End-to-end distance of single polymer chain observed by super-resolution method. ► Observation accuracy better than 1 nm for end-to-end distance. ► First experimental result of distribution of chain dimension in ultra-thin film. ► Negligible effect of confinement in ultra-thin film on lateral chain dimension.