Heteronuclear Adiabatic Relaxation Dispersion (HARD) for quantitative analysis of conformational dynamics in proteins

► Heteronuclear Adiabatic Relaxation Dispersion (HARD) detect fast and slow conformational dynamics. ► Adiabatic relaxation can be interpreted quantitatively. ► HARD method is complementary to CPMG and rotating frame spin-lock R1ρ relaxation measurements.