کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5406646 1393183 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNP enhanced frequency-selective TEDOR experiments in bacteriorhodopsin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
DNP enhanced frequency-selective TEDOR experiments in bacteriorhodopsin
چکیده انگلیسی
We describe a new approach to multiple 13C-15N distance measurements in uniformly labeled solids, frequency-selective (FS) TEDOR. The method shares features with FS-REDOR and ZF- and BASE-TEDOR, which also provide quantitative 15N-13C spectral assignments and distance measurements in U-[13C,15N] samples. To demonstrate the validity of the FS-TEDOR sequence, we measured distances in [U-13C,15N]-asparagine which are in good agreement with other methods. In addition, we integrate high frequency dynamic nuclear polarization (DNP) into the experimental protocol and use FS-TEDOR to record a resolved correlation spectrum of the Arg-13Cγ-15Nε region in [U-13C,15N]-bacteriorhodopsin. We resolve six of the seven cross-peaks expected based on the primary sequence of this membrane protein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetic Resonance - Volume 202, Issue 1, January 2010, Pages 9-13
نویسندگان
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