Fast acquisition of multi-dimensional spectra in solid-state NMR enabled by ultra-fast MAS

As a result, optimal 13C spectra of the oxidized, paramagnetic form of human copper zinc superoxide dismutase (SOD) can be obtained employing rf-fields which do not exceed 40 kHz during the whole experiment. This enables the removal of unwanted heating which can lead to deterioration of the sample. Furthermore, combined with the short 1H T1s, this allows the repetition rate of the experiments to be shortened from 3 s to 500 ms, thus compensating for the sensitivity loss due to the smaller sample volume in a 1.3 mm rotor. The result is that 2D 13C-13C correlation could be acquired in about 24 h on less than 1 mg of SOD sample.