کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5434365 1509141 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing: Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria
ترجمه فارسی عنوان
خواص ضد چسبندگی پیشرفته چند لایه پلی اتیلل الکترولیت اسید کیتوزان / هیالورونیک تحت هدایت حرارتی: ضعیف بودن سلول های پستانداران و کاهش انتخاب چسبندگی باکتری های گرم مثبت
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


- Thermal annealing enhances antifouling properties in chitosan/hyaluronate multilayers.
- Topography changes induced by the thermal annealing affect the antifouling characteristics of the multilayers.
- The increase in anti-adhesive properties is observed for different cell lines.
- Selective anti-adhesion for S. aureus bacteria is observed after thermal annealing.

The development of antifouling coatings with restricted cell and bacteria adherence is fundamental for many biomedical applications. A strategy for the fabrication of antifouling coatings based on the layer-by-layer assembly and thermal annealing is presented. Polyelectrolyte multilayers (PEMs) assembled from chitosan and hyaluronic acid were thermally annealed in an oven at 37 °C for 72 h. The effect of annealing on the PEM properties and topography was studied by atomic force microscopy, ζ-potential, circular dichroism and contact angle measurements. Cell adherence on PEMs before and after annealing was evaluated by measuring the cell spreading area and aspect ratio for the A549 epithelial, BHK kidney fibroblast, C2C12 myoblast and MC-3T3-E1 osteoblast cell lines. Chitosan/hyaluronic acid PEMs show a low cell adherence that decreases with the thermal annealing, as observed from the reduction in the average cell spreading area and more rounded cell morphology. The adhesion of S. aureus (Gram-positive) and E. coli (Gram-negative) bacteria strains was quantified by optical microscopy, counting the number of colony-forming units and measuring the light scattering of bacteria suspension after detachment from the PEM surface. A 20% decrease in bacteria adhesion was selectively observed in the S. aureus strain after annealing. The changes in mammalian cell and bacteria adhesion correlate with the changes in topography of the chitosan/hyaluronic PEMs from a rough fibrillar 3D structure to a smoother and planar surface after thermal annealing.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 80, 1 November 2017, Pages 677-687
نویسندگان
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