کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5434787 1509142 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of residual composition on the structure and properties of extracellular matrix derived hydrogels
ترجمه فارسی عنوان
اثر ترکیب باقی مانده بر ساختار و خواص هیدروژل های ماتریس خارج سلولی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


- The ECM components in hydrolysates from four decellularized tissues are established.
- Collagen polymerization and entanglement are promoted by the residual biomolecules.
- Residual macromolecules are crucial for the adaptation of the ECM gel properties.
- In vitro macrophage response is accentuated in hydrogels rich in ECM molecules.
- Each structure parameter can be harnessed to predict ECM gel behavior.

In this work, hydrolysates of extracellular matrix (hECM) were obtained from rat tail tendon (TR), bovine Achilles tendon (TAB), porcine small intestinal submucosa (SIS) and bovine pericardium (PB), and they were polymerized to generate ECM hydrogels. The composition of hECM was evaluated by quantifying the content of sulphated glycosaminoglycans (sGAG), fibronectin and laminin. The polymerization process, structure, physicochemical properties, in vitro degradation and biocompatibility were studied and related to their composition. The results indicated that the hECM derived from SIS and PB were significantly richer in sGAG, fibronectin and laminin, than those derived from TAB and TR. These differences in hECM composition influenced the polymerization and the structural characteristics of the fibrillar gel network. Consequently, the swelling, mechanics and degradation of the hydrogels showed a direct relationship with the remaining composition. Moreover, the cytocompatibility and the secretion of transforming growth factor beta-1 (TGF-β1) by macrophages were enhanced in hydrogels with the highest residual content of ECM biomolecules. The results of this work evidenced the role of the ECM molecules remaining after both decellularization and hydrolysis steps to produce tissue derived hydrogels with structure and properties tailored to enhance their performance in tissue engineering and regenerative medicine applications.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 79, 1 October 2017, Pages 793-801
نویسندگان
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