A novel route to prepare a multilayer system via the combination of interface-mediated catalytic chain transfer polymerization and thiol-ene click chemistry

- The poly(MMA) brush with vinyl end-group on the silicon substrate was first synthesized by the CCTP technique.
- The brush was then modified with biotin, streptavidin and protein-A, respectively.
- This multilayer system was used to attach the anti-IgG molecules in a highly oriented manner.

Herein, we have designed a novel multilayer system composed of poly(methyl methacrylate) [poly(MMA)] brush, biotin, streptavidin and protein-A on a silicon substrate to attach on anti-immunoglobulin G (anti-IgG). poly(MMA) brush with vinyl end-group was first synthesized by the interface-mediated catalytic chain transfer polymerization. The brush was then modified with cysteamine molecules to generate the polymer chains with amine end-group via a thiol-ene click chemistry. The amine end-groups of poly(MMA) chains were also modified with biotin units to ensure selective connection points for streptavidin molecules. Finally, a multilayer system on the silicon substrate was formed by using streptavidin and protein-A molecules, respectively. This multilayer system was employed to attach anti-IgG molecules in a highly oriented manner and provide anti-IgG molecular functional configuration on the multilayer. High reproducibility of the amount of anti-IgG adsorption and homogeneous anti-IgG adsorption layer on the silicon surface could be provided by this multilayer system. The multilayer system with protein A may be opened the door for designing an efficient immunoassay protein chip.

A novel route to the preparation of a multilayer system via the combination of catalytic chain transfer polymerization and thiol-ene click chemistry.70