کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5435162 1509145 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
TiO2 nanorod arrays modified Ti substrates promote the adhesion, proliferation and osteogenic differentiation of human periodontal ligament stem cells
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
TiO2 nanorod arrays modified Ti substrates promote the adhesion, proliferation and osteogenic differentiation of human periodontal ligament stem cells
چکیده انگلیسی


- TiO2 nanorod arrays (TNRs) was fabricated onto Ti substrates using hydrothermal and sintering method.
- TNRs had great potential in promoting the osteogenic activity of implants and the bone-implant integration.
- TNRs effectively promoted the adhesion, proliferation and osteogenic differentiation of hPDLSCs in vitro.

Nanostructure coating on titanium (Ti) implants is well known as a cue for directing osteoblast behavior and function. However, effects of nanostructure coatings on dental stem cells have been rarely explored. In this work, assembled TiO2 nanorod arrays (TNRs) were fabricated on the polished Ti substrates using hydrothermal and sintering methods. The adhesion, morphology, proliferation and osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) seeded onto TNRs substrates were evaluated. Ti substrates were used as control. Rougher TNRs showed better hydrophilicity and protein adsorption capacity compared with Ti control. When seeded on TNRs substrates, PDLSCs exhibited more stretched morphology and higher proliferation rate. Cytoskeletal F-actin expression was markedly promoted for PDLSCs cultured on TNRs substrates under osteogenic induction. Alkaline phosphatase (ALP) activity and mineral deposition were also enhanced by TNRs. Moreover, osteogenesis-related markers of ALP, runt related transcription factor 2 (Runx2) and osteopontin (OPN) of PDLSCs cultured on TNRs substrates were significantly up-regulated at both gene and protein levels when compared to Ti substrates. In conclusion, the unique structure of TNRs provided a biocompatible platform for modulating morphology and function of PDLSCs. The promotion of osteogenic differentiation indicated that the surface modification of implants with TNRs may improve the osteogenic activity of implants and the bone-implant integration in future clinical applications.

280

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 76, 1 July 2017, Pages 684-691
نویسندگان
, , , , , ,