کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5488778 | 1399584 | 2017 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrosynthesized poly(1,5-diaminonaphthalene)/polypyrrole nanowires bilayer as an immunosensor platform for breast cancer biomarker CA 15-3
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موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
فیزیک ماده چگال
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this paper, an electrosynthesized bilayer film of polypyrrole nanowire and poly(1,5diaminonaphthalene) on a disposable screen-printing carbon ink electrode is presented. The inner polypyrrole nanowire layer had a large surface-to-volume ratio and high conductivity in the neutral medium, whereas the outer layer of poly(1,5diaminonaphthalene) had functional amino groups on the polymer chains. The combination of polypyrrole nanowire and poly(1,5diaminonaphthalene) showed a promising material for electrochemical biosensing. Here we reported an electrochemical immunosensor based on this approach for the purpose of detecting breast cancer biomarkers. The bilayer could enhance the surface coverage of antibody anti-CA 15-3 and consequently improve the sensitivity and stability of the immunosensors. The magnetic beads were used as carriers of labeled antibody anti-CA15-3 and HRP (horseradish peroxidase) in order to achieve an amplification of the signal. Under optimized conditions, the linear range of the immunoassay was 0.05-20Â UÂ mLâ1 with a detection limit of 0.02Â UÂ mLâ1Â CA 15-3 antigen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Applied Physics - Volume 17, Issue 11, November 2017, Pages 1422-1429
Journal: Current Applied Physics - Volume 17, Issue 11, November 2017, Pages 1422-1429
نویسندگان
Vân-Anh Nguyen, Huy L. Nguyen, Dzung T. Nguyen, Quan P. Do, Lam D. Tran,