کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5501574 | 1534850 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Age-related changes in skeletal muscle composition: A pilot nuclear magnetic resonance spectroscopy study in mice
ترجمه فارسی عنوان
تغییرات مرتبط با سن در ترکیب عضله اسکلت: یک مطالعه اسپکتروسکوپی رزونانس مغناطیسی خلبان در موش
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
چکیده انگلیسی
The composition of skeletal muscle was investigated in the quadriceps and gastrocnemius muscle of 13-month-old (n = 15) and 23-month-old (n = 19) mice by means of high-resolution nuclear magnetic resonance (NMR) spectroscopy. Muscle specimens were dissected out, frozen in liquid nitrogen and extracted in chloroform/methanol, and proton NMR spectra of the resulting aqueous and organic fractions were obtained at 600 MHz. Several metabolites were unambiguously identified and quantified. Multivariate ANOVA (factor: age, muscle, age Ã muscle) showed a significant main effect of age (P = 0.031) on the amount of muscle metabolites, suggesting that the aging process affects the composition of skeletal muscle. Univariate tests showed significant differences for lactate, acetate, taurine, and uridine in 13- and 23-month-old mice. A trend for the effect of muscle (quadriceps vs. gastrocnemius; P = 0.128) was also found. No significant muscle x age interaction was present. When the same data were used in principal component analysis, the first two principal components separated muscles (quadriceps and gastrocnemius) and ages (13- and 23-month-old), explaining 66.7% of total variance. The results of this pilot study show that high-resolution NMR spectroscopy is able to detect age-associated changes in skeletal muscle metabolites, thereby paving the way to future detailed metabolomics investigation in sarcopenia of aging.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Gerontology - Volume 92, June 2017, Pages 23-27
Journal: Experimental Gerontology - Volume 92, June 2017, Pages 23-27
نویسندگان
Anatoly P. Sobolev, Luisa Mannina, Manuela Costanzo, Barbara Cisterna, Manuela Malatesta, Carlo Zancanaro,