کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5507043 1536897 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Denaturation process of laccase in various media by refractive index measurements
ترجمه فارسی عنوان
فرایند دوناتاسیون لاکتوز در رسانه های مختلف با اندازه گیری شاخص های شکست
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- Refractive index measurements to follow the denaturation process in various medium.
- The denaturation process was studied by using the Gladstone-Dale and the Lorentz-Lorentz models.
- [Morph][F] denatures laccase faster than [pyrr][F].

In this work, we are interested in the denaturation process of a laccase from Tramates versicolor via the determination of the refractive index, the refractive index increment and the specific volume in various media. The measurements were carried out using an Abbe refractometer. We have shown that the refractive index increment values obtained from the slope of the variation of the refractive index vs. Concentration are outside the range refractive index increments of proteins. To correct the results, we have followed the theoretical predictions based on the knowledge of the protein refractive index from its amino acids composition. The denaturation process was studied by calculating the specific volume variation where its determination was related to the Gladstone-Dale and the Lorentz-Lorentz models.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 11, September 2017, Pages 19-26
نویسندگان
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