کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5510640 1539271 2017 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The detoxification responses, damage effects and bioaccumulation in the scallop Chlamys farreri exposed to single and mixtures of benzo[a]pyrene and chrysene
ترجمه فارسی عنوان
واکنش سم زدایی، اثرات آسیب و ذخیره زیستی در گل کلم فرسری اسکالوپ در معرض یکنواخت و مخلوطی از بنزو پریئن و کرایسیسین
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
This study aimed to investigate the detoxification responses, damage effects and biotransformation in scallop Chlamys farreri exposed to benzo[a]pyrene (BaP) (0.1, 1 μg/L), chrysene (CHR) (0.1, 1 μg/L) and BaP + CHR (0.1 + 0.1, 1 + 1 μg/L) for 15 days. Results demonstrated that BaP and CHR concentration (BaP < CHR) in tissues increased rapidly in a time and dose effect. The mRNA expression of aryl hydrocarbon receptor (AhR), cytochrome P450 1A1 (CYP1A1), CYP1B1, multidrug resistance protein 1 (MRP1/ABCC1), breast cancer resistance protein (BCRP/ABCG2) and P-glycoprotein (P-gp) were induced especially in the mixtures of BaP and CHR. Heat shock protein 90 (HSP90) and aryl hydrocarbon receptor nuclear translocator (ARNT) mRNA expression was significantly elevated at days 1, 10 and 15. Detoxification enzymes of 7-ethoxyresorufin O-deethylase (EROD), uridine-diphosphate-glucuronyl-transferase (UGT) and sulfotransferase (SULT) were significantly induced and then became stable gradually while glutathione-S-transferase (GST) was inhibited in the mixtures of BaP and CHR at days 10 and 15. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH) were all stimulated especially in the mixtures of BaP and CHR. The levels of DNA strand breaks, lipid peroxidation (LPO) and protein carbonyl (PC) contents showed damage effects exposed BaP and CHR. All the results indicated that BaP and CHR have similar induced effect and a majority of the biomarkers pointed to a more toxic effect when BaP and CHR were mixed. These will provide a solid foundation for the study of PAHs detoxification mechanism in bivalves and valuable information for marine pollution monitoring.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 191, January 2017, Pages 36-51
نویسندگان
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