کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5511638 | 1540214 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of keratin microparticles from feather biomass with potent antioxidant and anticancer activities
ترجمه فارسی عنوان
تعیین خصوصیات میکروپارسیت های کراتین از بیوماس پر از فعالیت های آنتی اکسیدانی و ضد سرطانی
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
پر مرغ، کراتین، میکرو ذرات، ضد اکسید کننده، ضد سرطان، نقطه ایزوالکتریک،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
In the present study chicken feathers were hydrolyzed by chemical treatment in alkaline conditions. The pH value of feather hydrolyzed solution was amended accordingly the iso-electric precipitation. Two types of keratin microparticles KM1, KM2 were synthesized under acidic conditions at 3.5 and 5.5 pH respectively. The synthesized keratin microparticles possessed uniform and round surface by scanning electron microscopy (SEM). The thermal degradation of microparticles were examined by thermogravimetry (TGA). Fourier transform infrared spectroscopy (FTIR) revealed that the extracted keratin retained the most of protein backbone. The microparticles were screened for their in vitro anticancer activities by SRB bioassay towards HeLa, SK-OV-3 and A549 cancer cell lines. Futhermore, their cytotoxicity towards healthy cell lines was analyzed having Malin Darby canine kidney (MDCK) cell lines along with in vitro antioxidant activity using DPPH and ABTS methods KM1 and KM2 showed 200.31 ± 1.01 and 139.73 ± 0.94, 214.16 ± 0.29 and 153.92 ± 0.61, 328.92 ± 3.46 and 200.33 ± 2.48 μg/mL of IC50 levels against HeLa, SK-OV-3, and A549 cell lines, respectively. Moreover, KM1 and KM2 demonstrated significant antioxidant potency with IC50 levels 13.15 and 9.02 μg/mL as well as 8.96 and 5.60 μg/mL in DPPH and ABTS radical scavenging bioassay, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 104, Part A, November 2017, Pages 189-196
Journal: International Journal of Biological Macromolecules - Volume 104, Part A, November 2017, Pages 189-196
نویسندگان
Swati Sharma, Arun Gupta, Syed Mohd S.T. Chik, Chua Geek Kee, Bhupendra M. Mistry, Doo H. Kim, Gaurav Sharma,