کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5512472 | 1540225 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phosphorylated Codonopsis pilosula polysaccharide could inhibit the virulence of duck hepatitis A virus compared with Codonopsis pilosula polysaccharide
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کلمات کلیدی
DEHsPBSDMEMDHAVVirus controlSTMPDVHIFN-β3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromideDuck hepatitis A virusSTPPCPPs - CPP هاMTT - MTTReal-time PCR - PCR به موقعagarose gel electrophoresis - الکتروفورز ژل آگارزSodium trimetaphosphate - تری متیل فسفات سدیمSodium tripolyphosphate - تریپلی فسفات سدیمAge - سنAntiviral activity - فعالیت ضد ویروسیMaintenance medium - متوسط نگهداریPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده Dulbeccoreal-time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیcell control - کنترل سلول
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To screen effective anti-duck hepatitis A virus (DHAV) drugs, we applied STMP-STPP method to prepare phosphorylated Codonopsis pilosula polysaccharide (pCPPS), the phosphorylation-modified product of Codonopsis pilosula polysaccharide (CPPS). The IR spectrum and field emission scanning electron microscope (FE-SEM) were subsequently used to analyze the structure of pCPPS. Several tests were conducted to compare the anti-DHAV activities of CPPS and pCPPS. The MTT method was used to compare the effect of the drugs on DHAV-infected duck embryonic hepatocytes (DEHs), and the Reed-Muench assay was employed to observe changes in the virulence of DHAV. We also applied real-time PCR to examine the relationship between virus replication and the expression of IFN-β. The results indicated that CPPS could not inhibit the replication of DHAV. In contrast, pCPPS increased the virus TCID50, inhibited viral replication and, accordingly, increased the survival rate of DEHs infected with DHAV. Because DHAV induced the expression of IFN-β, and the IFN-β expression level was positively associated with the number of DHAV, the reduction of IFN-β expression levels after pCPPS treatment demonstrated a decrease in the number of virus particles. These results indicated that pCPPS, which reduces the number of DHAV, was more effective than CPPS in anti-DHAV activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 94, Part A, January 2017, Pages 28-35
Journal: International Journal of Biological Macromolecules - Volume 94, Part A, January 2017, Pages 28-35
نویسندگان
Ke Ming, Yun Chen, Fangke Yao, Jintong Shi, Jingjing Yang, Hongxu Du, Xunyi Wang, Yixuan Wang, Jiaguo Ph.D,