کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5514775 | 1541694 | 2016 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intraperitoneal injection urocortin-3 reduces the food intake of Siberian sturgeon (Acipenser baerii)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CARTqPCRORFCCKCRFALANPYAgRPPOMCi.p.alanine - آلانینOrexins - اورکسین هاSER - برای بودنrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدbase pairs - جفت پایهintraperitoneal - داخل صفاقیSerine - سرینcorticotropin releasing factor - عامل آزاد کننده کورتیکوتروپینopen reading frame - قاب خواندن بازRace - مسابقهmya - میاmillion years ago - میلیون سال پیشquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیbody weight - وزن بدنproopiomelanocortin - پروپیوملانوکورتینhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراcholecystokinin - کولهسیستوکینینcocaine and amphetamine regulated transcript - کوکائین و رونویسی تنظیم شده توسط آمفتامینNeuropeptide Y - یوروپروتئین Y
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Urocortin-3 (UCN3), one of the corticotropin releasing factor (CRF) family peptides, which was discovered in 2001, has a variety of biological functions. However, the researches of UCN3 in fish were scarce. In order to understand whether UCN3 play a role in regulating food intake in fish, we first cloned the ucn3 cDNAs sequence of Siberian sturgeon (Acipenser baerii Brandt), and investigated the ucn3 mRNA levels in 11 tissues. The Siberian sturgeon ucn3 cDNA sequence was 1044 bp, including an open reading frame (ORF) of 447 bp that encoded 148 amino acids with a mature peptide of 40 amino acids, a 5ʹ-terminal untranslated region (5ʹ-UTR) of 162 bp and a 3ʹ-terminal untranslated region (3ʹ-UTR) of 435 bp. The result of tissue distribution showed that ucn3 widely distributed in 11 tissues with highest expression in brain. We also assessed the effects of periprandial (pre- and post-feeding), fasting and re-feeding on ucn3 mRNAs abundance in brain. The results showed the expression of ucn3 mRNA in brain was significantly elevated after feeding, decreased after fasting 17âdays and increased after re-feeding. To further investigate the food intake role of UCN3 in Siberian sturgeon, we performed intraperitoneal (i.p.) injection of Siberian sturgeon UCN3 (SsUCN3) with three doses (60, 120 or 240 ng/g) and recorded the food intake. Acute and chronic i.p. injection SsUCN3 reduced the food intake in a dose-dependent pattern. In conclusion, this study indicates that SsUCN3 acts as a satiety factor to inhibit the food intake of Siberian sturgeon.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 85, November 2016, Pages 80-88
Journal: Peptides - Volume 85, November 2016, Pages 80-88
نویسندگان
Xin Zhang, Yuanbing Wu, Jin Hao, Jieyao Zhu, Ni Tang, Jinwen Qi, Shuyao Wang, Hong Wang, Shuang Peng, Ju Liu, Yundi Gao, Defang Chen, Zhiqiong Li,