کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5516674 | 1542684 | 2017 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Screening for strains with 11α-hydroxylase activity for 17α-hydroxy progesterone biotransformation Screening for strains with 11α-hydroxylase activity for 17α-hydroxy progesterone biotransformation](/preview/png/5516674.png)
- The biotransformation of 17α-OH-progesterone has been studied.
- 11α,17α-diOH-Progesterone can be hydroxylated by Cunninghamella elegans CICC40250 efficiently.
- High substrate concentration (30Â g/L) and high yield were obtained by adding coenzyme precursor glucose.
Various corticosteroids are prepared by using 11α,17α-diOH-progesterone (11α,17α-diOH-PROG) as an important intermediate and raw material. Hence, strains that can improve the yields of 11α,17α-diOH-PROG should be screened. Cunninghamella elegans CICC40250 was singled out from five common 11α hydroxylation strains. The reaction parameters of 11α,17α-diOH-PROG production were also investigated. C. elegans CICC40250 could efficiently catalyze the hydroxylation of 17α-hydroxy progesterone (17α-OH-PROG) at C-11α position. This strain could also effectively convert 11α,17α-diOH-PROG at high substrate concentrations (up to 30 g/L). After the coenzyme precursor glucose was added, the rate of 11α,17α-diOH-PROG formation reached 84.2%, which was 11.4% higher than that of the control group. Our study established a simple and feasible mechanism to increase 11α,17α-diOH-PROG production levels. This mechanism involves C. elegans CICC40250 that can be efficiently applied to induce the biotransformation of 17α-OH-PROG with a hydroxylation biocatalytic ability.
Journal: Steroids - Volume 124, August 2017, Pages 67-71