کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5516744 | 1542692 | 2016 | 4 صفحه PDF | دانلود رایگان |
- An improved methodology for high-yielding stereoselective hydroxylation of canrenone was developed.
- The use of O2-enriched air proved to be very effective for microbial hydroxylation of steroids in a stirred tank reactor.
- The control of dissolved oxygen is a key-point in controlling microbial steroid hydroxylation.
A high yielding bioprocess for 11-α hydroxylation of canrenone (1a) using Aspergillus ochraceus ATCC 18500 was developed. The optimization of the biotransformation involved both fermentation (for achieving highly active mycelium of A. ochraceus) and biotransformation with the aim to obtain 11-α hydroxylation with high selectivity and yield. A medium based on sucrose as C-source resulted particularly suitable for conversion of canrenone into the corresponding 11-hydroxy derivative, whereas the use of O2-enriched air and dimethyl sulfoxide (DMSO) as a co-solvent for increasing substrate solubility played a crucial role for obtaining high yields (>95%) of the desired product in high chemical purity starting from 30 mM (10.2 g/L) of substrate. The structure of the hydroxylated product was confirmed by a combination of two-dimensional NMR proton-proton correlation techniques.
Journal: Steroids - Volume 116, December 2016, Pages 1-4