کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5517960 1543861 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Review articleFunctional proteomics of light-harvesting complex proteins under varying light-conditions in diatoms
ترجمه فارسی عنوان
بررسی پروتئومیکای کمپلکس کم نور در شرایط مختلف نور در دیاتومه
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
چکیده انگلیسی

Comparative proteome analysis of subcellular compartments like thylakoid membranes and their associated supercomplexes can deliver important in-vivo information on the molecular basis of physiological functions which go far beyond to that what can be learnt from transcriptional-based gene expression studies. For instance, the finding that light intensity influences mainly the relative stoichiometry of subunits could be obtained only by high resolution proteome analysis. The high sensitivity of LC-ESI-MS/MS based proteome analysis allows the determination of proteins in very small subfractions along with their non-labeled semi quantitative analysis. This provides insights in the protein-protein interactions of supercomplexes that are the operative units in intact cells. Here, we have focused on functional proteome approaches for the identification of microalgal light-harvesting complex proteins in chloroplasts and the eyespot in general and in detail for those of diatoms that are exposed to varying light conditions

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 217, October 2017, Pages 38-43
نویسندگان
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