RNA editing of cytochrome c maturation transcripts is responsive to the energy status of leaf cells in Arabidopsis thaliana

- AtPAP2 interacts with all MORF proteins except MORF4 and MORF7.
- We identified 34 and 510 RNA editing sites in chloroplast transcripts and mitochondrial transcripts, respectively.
- Some sites in the transcripts of several cytochrome c maturation (Ccm) transcripts were differentially edited.
- Western blotting of 2D BN-PAGE showed that the patterns of CcmFN1 polypeptides were different between the lines.
- We proposed AtPAP2 may influence cytochrome c biogenesis by modulating RNA editing through its interaction with MORF proteins.

Overexpression of AtPAP2, a phosphatase located on the outer membranes of chloroplasts and mitochondria, leads to higher energy outputs from these organelles. AtPAP2 interacts with seven MORF proteins of the editosome complex. RNA-sequencing analysis showed that the editing degrees of most sites did not differ significantly between OE and WT, except some sites on the transcripts of several cytochrome c maturation (Ccm) genes. Western blotting of 2D BN-PAGE showed that the patterns of CcmFN1 polypeptides were different between the lines. We proposed that AtPAP2 may influence cytochrome c biogenesis by modulating RNA editing through its interaction with MORF proteins.