کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5520194 | 1544692 | 2017 | 7 صفحه PDF | دانلود رایگان |
- We assessed the role of Kp-10 in bovine granulosa cells.
- Kp-10 was expressed in the cytoplasm of bovine ovarian granulosa cells.
- Kp-10 inhibited proliferation and promoted apoptosis in ovarian granulosa cells.
Previous studies have shown that kisspeptin (Kp-10) is expressed in mammalian ovaries; however, the expression and role of Kp-10 in bovine ovarian granulosa cells are still unclear. In this study, we assessed the expression of Kp-10 and its effects on the proliferation and apoptosis of bovine granulosa cells. Immunohistochemical analysis showed that Kp-10 was expressed in the cytoplasm of bovine ovarian granulosa cells. Moreover, MTT assays showed that 100Â nM Kp-10 significantly inhibited the viability of granulosa cells (PÂ <Â 0.05). Flow cytometry analysis showed that Kp-10 could significantly increase accumulation of cells in the G1 phase, decrease accumulation of cells in the S phase, and promote apoptosis in bovine granulosa cells (PÂ <Â 0.05). Additionally, Kp-10 decreased the mRNA levels of Bcl-2, an anti-apoptotic gene; increased the mRNA levels of caspase-3, a pro-apoptotic gene; and increased the mRNA levels of Fas and Fasl (PÂ <Â â0.05). Thus, our findings demonstrated for the first time that Kp-10 inhibited proliferation and promoted apoptosis in bovine ovarian granulosa cells. These findings provide insights into our understanding of the role of Kp-10 in mediating the proliferation of bovine granulosa cells.
Journal: Animal Reproduction Science - Volume 185, October 2017, Pages 1-7