کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5520848 | 1401233 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Creation of targeted inversion mutations in plants using an RNA-guided endonuclease
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Creation of targeted inversion mutations in plants using an RNA-guided endonuclease Creation of targeted inversion mutations in plants using an RNA-guided endonuclease](/preview/png/5520848.png)
چکیده انگلیسی
Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases (RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were 2.6% and 2.2% in the Arabidopsis FLOWERING TIME (AtFT) and TERMINAL FLOWER 1 (AtTFL1) loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Crop Journal - Volume 5, Issue 1, February 2017, Pages 83-88
Journal: The Crop Journal - Volume 5, Issue 1, February 2017, Pages 83-88
نویسندگان
Congsheng Zhang, Changlin Liu, Jianfeng Weng, Beijiu Cheng, Fang Liu, Xinhai Li, Chuanxiao Xie,