Current state and latest advances in the concept, production and functionality of prebiotic oligosaccharides

- Prebiotic definition could change in the future with the aim to broad the concept.
- Protein engineering tools are useful to make novel enzymes with glycosidase activity.
- Seaweeds and marine microalgae are promising sources for prebiotic oligosaccharides.
- Future research will focus on well-designed prebiotic dietary interventions.
- Research needed on underlying mechanisms of action and on human intervention studies.

The increasing knowledge on the complexity of the gut microbiota and its interaction with the host is challenging the concept and definition of prebiotics. This review emphasises the recent advances in the enzymatic production of prebiotic oligosaccharides, the use of renewable and/or alternative sources, that is seaweeds and marine microalgae, and the clinical evidences towards the beneficial effects of prebiotic oligosaccharides in the gut and other systems. It also focuses on the current gaps and research needs in the field of prebiotics, highlighting the necessity to gain a deeper knowledge on mechanisms that govern the interactions between prebiotics and human gut microbiota, on chemical structure-function relationship of prebiotics or to develop well-controlled human intervention studies to support claims of health benefits.