کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5521427 | 1545305 | 2017 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Research paperEstablishment and characterization of an in vitro human small airway model (SmallAirâ¢) Research paperEstablishment and characterization of an in vitro human small airway model (SmallAirâ¢)](/preview/png/5521427.png)
We report here the establishment and characterization of an in vitro human small airway model (SmallAirâ¢). The epithelial cells were isolated from the distal lungs by enzymatic digestion. After amplification, the cells were seeded on the microporous membrane of Transwell inserts. Once confluent, the cultures were switched to air-liquid interface. After 3 weeks of culture, the epithelium became fully differentiated, with morphology of columnar epithelium, and a thickness of 10-15 μm. Most significantly, CC-10, a specific marker of Club cells, was highly expressed in SmallAirâ¢. CC-10 was detected by both immune-cytochemistry and Western Blot. As expected, SmallAir⢠contained few Muc5-Ac positive cells (goblet cells). In contrast, CC-10 was not detected in MucilAirâ¢, an in vitro model of the human nasal and bronchial epithelial model. Instead, Muc5-Ac was highly expressed in MucilAirâ¢. However, both MucilAir⢠and SmallAir⢠contain basal cells and ciliated cells, showing cilia beating and mucociliary clearance. Clearly, MucilAir⢠and SmallAir⢠are two distinct airway epithelial models.
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Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 118, September 2017, Pages 68-72