کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5521768 1545527 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of high hydrostatic pressure on lipase from Rhizopus chinensis: I. Conformational changes
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Effects of high hydrostatic pressure on lipase from Rhizopus chinensis: I. Conformational changes
چکیده انگلیسی


- A framework to evaluate the influence of food processing on lipase was established using molecular modeling concept.
- A combination of the experiment and the simulation to investigate the structure-function relationship of enzyme was attempted.
- This work can help in evaluation of process parameters (like pressure) to modulate the protein functional properties.

Conformational changes of Rhizopus chinensis lipase (RCL) induced by high hydrostatic pressure processing, were evaluated by molecular dynamic (MD) simulations and experimental methods. In most lipases a lid covers the substrate binding site keeping the lipase active when the lid is open, whereas the lipase is inactive when the lid is close. Results of this research showed that lid was closed when high pressures in the range 400-600 MPa were applied, whereas it was open at pressures around 200 MPa, at which the lipase exhibited a high activity. Simulations and Circular dichroism (CD) tests confirmed that the lid length kept constant at all pressures. At pressures around 200 MPa, hydrogen bonds between the amino acids Ser145 and His257 was preserved whereas at pressures around 600 MPa, hydrogen bonds between the amino acids His257 and Asp204 was significantly weakened Docking studies suggested that the highest and lowest binding affinity between the enzyme active site and the substrate occurred when pressures of 200 and 600 MPa, were applied, respectively.The enzyme tertiary structure changed significantly at pressures higher than 500 MPa. The presence of the amino-acid Lys202 near the active site plays a key role in determining the fluorescence properties and fluorescence spectra of RCL. MD simulations confirmed that was the reason for changes in fluorescence observed at pressures over 200 MPa.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Innovative Food Science & Emerging Technologies - Volume 41, June 2017, Pages 267-276
نویسندگان
, , , , , ,