کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5523476 1546078 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of female bovine plasma collected at different days of the estrous cycle on epididymal spermatozoa motility
ترجمه فارسی عنوان
اثر پلاسمایی گاو ماده جمع آوری شده در روزهای مختلف چرخه استروئید بر تحرک اسپرم های اپیدیدیمال
کلمات کلیدی
اسپرم، زیرمجموعه ها، گاو، تحریک حرکتی، پلاسما،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


- Male, female or charcoal-treated plasma activated significantly bovine sperm motility.
- Three sperm subpopulations were identified, SP1: poorly motile, SP2: vigorous and SP3: non-vigorous but linear.
- SP2 subpopulation, was most present and more maintained over time in female plasma.
- Sperm activation was cycle-stage dependent, more marked in late stages.
- The cycle-stage dependent effect suggests an important role of progesterone.

The aim of this study was to assess the effects of female bovine plasma collected at different days of the reproductive cycle on epididymal spermatozoa motility and to test hypothesis that the subpopulations pattern of motile spermatozoa is affected by this treatment. Blood plasma samples were collected from five Holstein Friesian cows at different stages of the estrous cycle (days 0, 5, 10, 12 and 18), one pregnant cow and one adult bull and were diluted 1:9 (V/V) with normal saline. Female charcoal-treated plasma, Bull plasma and saline were used as controls. Semen samples were obtained from cauda epididymidis through retrograde flushing and diluted in saline to approximately 60 × 106 sperm/ml. The extended semen was diluted 1:2 (V/V) with tested media and motility was evaluated at 15 min and then every hour for 6 h using a computer-assisted semen analysis. Multivariate clustering procedure was applied to identify and quantify specific subpopulations within the semen samples. The statistical analysis clustered all the motile spermatozoa into three separate subpopulations with defined patterns of movement: Subpopulation 1 poorly motile and non-progressive spermatozoa (39.3%), subpopulation 2 including the fastest and the most vigorous spermatozoa (46.4%) and subpopulation 3 represented by slow, non-vigorous but linear spermatozoa (14.3%). Initially, sperm samples supplemented with female, male or female charcoal-treated plasma stimulated equally total motility and spermatozoa belonging to subpopulation 2 regardless of the estrous cycle stage. After 1-h incubation, the motility of these both categories of spermatozoa (total motile and those assigned to subpopulation 2) is enhanced and maintained more in day 12, 18 and pregnant cow plasma than in female plasma from earlier stage of the estrous cycle (day 0, 5 and 10), male plasma and female-charcoal treated plasma. In conclusion, the overall results showed that female plasma stimulated significantly sperm motility, especially at the late stage of the estrous cycle. Additionally, to the diverse compounds contained in blood plasma, progesterone may play a key role in such motility activation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 91, 15 March 2017, Pages 44-54
نویسندگان
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