کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5530632 1549383 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperLoss of WDFY3 ameliorates severity of serum transfer-induced arthritis independently of autophagy
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Research paperLoss of WDFY3 ameliorates severity of serum transfer-induced arthritis independently of autophagy
چکیده انگلیسی


- Loss of WDFY3 ameliorates disease severity in an arthritis animal model.
- WDFY3 is not essential in starvation-induced autophagy in myeloid cells.
- Loss of WDFY3 leads to decreased level of SQSTM1 in myeloid cells.

WDFY3 is a master regulator of selective autophagy that we recently showed to interact with TRAF6 and augment RANKL-induced osteoclastogenesis in vitro and in vivo via the NF-κB pathway. Since the NF-κB pathway plays a major role in inflammation herein, we investigate the role of WDFY3 in an arthritis animal model. Our data show that WDFY3 conditional knockout mice (Wdfy3loxP/loxP-LysM-Cre+) were protected in the K/BxN serum transfer-induced arthritis animal model. These effects were independent of alterations in starvation-induced autophagy as evidenced by Western blot analysis of the autophagy marker LC3, autophagosome formation in osteoclast precursors and lysosome formation in osteoclasts derived from WDFY3-cKO mice compared to controls. Moreover, we demonstrate by immunofluorescence and co-immunoprecipitation that WDFY3 interacts with SQSTM1 in macrophages and osteoclasts. Collectively, our data suggest that loss of WDFY3 in myeloid cells leads to reduced severity of inflammatory arthritis independently of WDFY3 function in starvation-induced autophagy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 316, June 2017, Pages 61-69
نویسندگان
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