کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5530690 1549387 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperCannabidiol (CBD) induces functional Tregs in response to low-level T cell activation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Research paperCannabidiol (CBD) induces functional Tregs in response to low-level T cell activation
چکیده انگلیسی


- CBD induces CD25 and FOXP3 on CD4+ T cells in response to Us/o, S/o and s3/28 stimulation.
- CBD is most effective in inducing CD4+CD25+FOXP3+ T cells in response to Us/o.
- Us/o + CBD increased FOXP3 expression on CD4+ cells derived from FOXP3-GFP mice.
- Us/o + CBD induced FOXP3 in pre-purified CD4+CD25− and CD4+CD25+ T cells.
- Us/o + CBD-induced CD4+CD25+ T cells suppressed responder cell proliferation.

Many effects of the non-psychoactive cannabinoid, cannabidiol (CBD), have been described in immune responses induced by strong immunological stimuli. It has also been shown that CBD enhances IL-2 production in response to low-level T cell stimulation. Since IL-2, in combination with TGF-β1, are critical for Treg induction, we hypothesized that CBD would induce CD4+CD25+FOXP3+ Tregs in response to low-level stimulation. Low-level T cell stimulation conditions were established based on minimal CD25 expression in CD4+ cells using suboptimal PMA/Io (4 nM/0.05 μM, S/o), ultrasuboptimal PMA/Io (1 nM/0.0125 μM, Us/o) or soluble anti-CD3/28 (400-800 ng each, s3/28). CBD increased CD25+FOXP3+ cells from CD4+, CD4+CD25+, and CD4+CD25− T cells, as well as in CD4+ T cells derived from FOXP3-GFP mice. Most importantly, the Us/o + CBD-induced CD4+CD25+ Tregs robustly suppressed responder T cell proliferation, demonstrating that the mechanism by which CBD is immunosuppressive under low-level T cell stimulation involves induction of functional Tregs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 312, February 2017, Pages 25-34
نویسندگان
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