کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5530842 1549444 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The role of global histone post-translational modifications during mammalian hibernation
ترجمه فارسی عنوان
نقش تغییرات هیستون پس از ترجمه در هنگام خواب زمستانی پستانداران
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


- Thirteen-lined ground squirrels employ hibernation to cope with extreme conditions.
- Hibernation entails significant changes in transcriptional control.
- Differential post-translational modification of histones occur throughout torpor.
- Mass spectrometry reveals histone modifications potentially important for torpor.
- These results warrant for deeper epigenetic investigations in I. tridecemlineatus.

Mammalian hibernators must cope with hypothermia, ischemia-reperfusion, and finite fuel reserves during days or weeks of continuous torpor. One means of lowering ATP demands during hibernation involves substantial transcriptional controls. The present research analyzed epigenetic regulatory factors as a means of achieving transcriptional control over cycles of torpor-arousal. This study analyzes differential regulation of select histone modifications (e.g. phosphorylation, acetylation, methylation), and identifies post-translational modifications on purified histones using mass spectrometry from thirteen-lined ground squirrels (Ictidomys tridecemlineatus). Post-translational modifications on histone proteins were responsive to torpor-arousal, suggesting a potential mechanism to dynamically alter chromatin structure. Furthermore, proteomic sequencing data of ground squirrel histones identified lysine 19 and 24 acetylation on histone H3, while acetylation sites identified on H2B were lysine 6, 47, 110, and 117. The present study provides a new glimpse into the epigenetic mechanisms which may play a role in transcriptional regulation during mammalian hibernation.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 75, April 2017, Pages 28-36
نویسندگان
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