کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5530851 1549444 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hypoxia enhances the viability, growth and chondrogenic potential of cryopreserved human adipose-derived stem cells
ترجمه فارسی عنوان
هیپوکسی باعث افزایش حیات، رشد و پتانسیل کاندیدایی سلول های بنیادی شده از انسان می شود.
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


- Cryopreserved ASCs show an increased viability in hypoxia.
- Hypoxia enhances the proliferation rate of cryopreserved ASCs.
- Hypoxia upregulates HIF-1α, which enhances chondrogenesis of cryopreserved ASCs.

Cryopreservation is the only existing method of storage of human adipose-derived stem cells (ASCs) for clinical use. However, cryopreservation has been shown to be detrimental to ASCs, particularly in term of cell viability. To restore the viability of cryopreserved ASCs, it is proposed to culture the cells in a hypoxic condition. To this end, we aim to investigate the effect of hypoxia on the cryopreserved human ASCs in terms of not only cell viability, but also their growth and stemness properties, which have not been explored yet. In this study, human ASCs were cultured under four different conditions: fresh (non-cryopreserved) cells cultured in 1) normoxia (21% O2) and 2) hypoxia (2% O2) and cryopreserved cells cultured in 3) normoxia and 4) hypoxia. ASCs at passage 3 were subjected to assessment of viability, proliferation, differentiation, and expression of stemness markers and hypoxia-inducible factor-1 alpha (HIF-1α). We found that hypoxia enhances the viability and the proliferation rate of cryopreserved ASCs. Further, hypoxia upregulates HIF-1α in cryopreserved ASCs, which in turn activates chondrogenic genes to promote chondrogenic differentiation. In conclusion, hypoxic-preconditioned cryopreserved ASCs could be an ideal cell source for cartilage repair and regeneration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 75, April 2017, Pages 91-99
نویسندگان
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